We utilize Escherichia coli as a platform for engineering transcription factors that detect small molecules. These transcription factors are expressed with their operator sequence preceding the DNA that encodes the Green Fluorescent Protein (GFP). Upon the addition of a ligand to the cells, the transcription factor dissociates from its operator sequence, thereby enabling the transcription and translation of GFP. By inducing mutations in the ligand-binding region of the transcription factor, we can generate variants that exhibit increased sensitivity to the target ligand. This project employs an integrated approach that includes screening, selections, computational analysis, and protein design.

Associated Lab Members:

Alia Clark-ElSayed

Charlie Johnson